How much is Taq polymerase?
Choose the Right DNA Polymerase for PCR
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What is Ex Taq polymerase?
Ex Taq DNA polymerase—a robust PCR enzyme with proofreading activity. Ex Taq polymerase has a higher fidelity than standard Taq with a mutation rate approximately 4.5 times lower, as determined by the Kunkel method. Ex Taq polymerase is supplied with optimized 10X buffer (with or without Mg2+) and dNTPs.
How long does Taq polymerase last?
TopTaq DNA Polymerase and the TopTaq Master Mix Kit are shipped on dry ice but retain full activity at room temperature (15-25°C) for at least 2 weeks. Both kits can be stored at 2-8°C for up to 12 months.
Is Taq polymerase harmful?
Inhalation : SureStart Taq DNA polymerase No known significant effects or critical hazards. SureStart Taq 10X Reaction Buffer No known significant effects or critical hazards.
Why is Taq polymerase special?
Taq DNA Polymerase is highly efficient, so it becomes fully functional as it reaches its optimum temperature. It also has a half-life of more than two hours (at a temperature of 92 °C), a high-amplification capacity, and the ability to add 150 nucleotides per second.
Does Taq polymerase denature DNA?
Taq polymerase has the important characteristic of being stable at temperatures up to 95°C2. That’s critical because this is the temperature at which DNA denatures – a required step at the beginning of the PCR reaction.
Why must Taq polymerase be kept cold?
Therefore, enzymes need to be kept cold at all times, even if you are just pipetting out of them quickly. This is particularly important when working with Taq for PCR reactions; the polymerase can start working immediately so it is important to add the enzyme and get your samples into the machine quickly.
Why does Taq polymerase not denature at 75 degrees?
This is due to the fact that during PCR the reactants are heated to 95°C and normal DNA Polymerase III would be denatured by this high temperature. The optimum temperature for the Taq polymerase is 75-80 degree Celsius.
What does Taq polymerase do to DNA?
“The function of Taq DNA polymerase in PCR is to amplify or synthesize DNA or gene of interest for various downstream applications. It’s a type of thermostable DNA polymerase, work at a higher temperature as well.”
Which is the last step in PCR?
The final stage is the extension step (20 sec to 1 min at 72 °C), which is performed so that the DNA polymerase extends the primer sequences from the 3′ of each primer to the end of the amplicon. A 1 min extension is typically sufficient to synthesize PCR fragments up to 2 kilobases (kb).
Why is Taq polymerase so special?
Taq makes DNA products that have A (adenine) overhangs at their 3′ ends. Also, Taq DNA Polymerase is the standard for routine PCR. It is “special” because it comes from the bacterium Thermus aquaticus, which lives in hot springs. So it is thermostable even at high temperatures, while other polymerases (eg E.
When to use Takara ex Taq DNA polymerase?
TaKaRa Ex Taq DNA Polymerase combines the proven performance of Takara Taq polymerase with the proofreading activity of an efficient 3′-to-5′ exonuclease, for high-sensitivity, high-efficiency PCR reactions. It can also be used for long-range PCR (up to 20 kb from genomic DNA templates and up to 30 kb from lambda DNA templates).
How big is the amplicon of Takara ex Taq?
Takara Ex Taq DNA polymerase combines the proven performance of Takara Taq polymerase with the proofreading activity of an efficient 3′-to-5′ exonuclease, for high-sensitivity, high-efficiency PCR. Ex Taq is optimized for amplicons up to 20 kb from genomic DNA, and up to 30 kb from lambda DNA.
Which is hot start version of Takara ex Taq?
A hot-start version of Takara Ex Taq DNA polymerase, which combines the proven performance of Takara Taq polymerase with the proofreading activity of an efficient 3′-to-5′ exonuclease, for high-sensitivity, high-efficiency PCR. Ex Taq is optimized for amplicons up to 20 kb from genomic DNA, and up to 30 kb from lambda DNA.
Which is PCR master mix contains ex Taq enzyme?
A 2X PCR master mix contains Ex Taq enzyme, reaction buffer (with Mg 2+ ), and dNTPs—for a simple PCR setup and minimal pipetting steps. A loading-dye added versions, PerfectShot Ex Taq DNA Polymerase, is also available for convenient loading of PCR products in agarose gels directly.