What is the importance of the Kia test in microbiology?
What is the importance of the Kia test in microbiology?
This medium became known as KIA. It is recommended for determination of H2S production by enteric gram-negative bacilli and for detection of H2S produced by some strains of Pseudomonas.
What does kligler iron agar test for?
Principle. Kligler Iron Agar (KIA) test is used in differentiating certain members of the Enterobacteriaceae by demonstrating hydrogen sulfide production and the fermentation of dextrose and lactose.
Which organism is positive for Kia?
Kligler’s Iron Agar (KIA) is used for the detection of carbohydrate fermentation. Reactions of KIA helps to include/exclude particular bacterial isolate in the family Enterobacteriaceae….Organism and their reaction in KIA.
| Name of the Organism | Pseudomonas aeruginosa |
|---|---|
| Reactions (Red= Alk, Yellow = Acid) | Alk/Alk |
| Gas | – |
| H2S | – |
What is a lactose test in microbiology?
What is the purpose of the test? The purpose is to see if the microbe can ferment the carbohydrate (sugar) lactose as a carbon source. How is lactose fermentation determined? If lactose is fermented to produce acid end products, the pH of the medium will drop.
How do I test my Kia?
Use of KIA:
- Used to differentiate Salmonella, Shigella and other enteria bacteria on the basis ofglucose and lactose fermentation and production of H2S gas.
- *Use a straight wire to inoculate KIA medium, first stabbing the butt and then streaking the slope in a zig-zag pattern.
What is the pH indicator in Kia?
The basal medium of KIA is composed of casein and meat peptones with the addition of lactose and dextrose. The production of acid by lactose- and/or dextrose-fermentation results in color changes of the phenol red pH indicator.
What does a Kia slant test for?
This is a differential medium. It tests for organisms’ abilities to ferment glucose and lactose to acid and acid plus gas end products. It also allows for identification of sulfur reducers.
Which bacteria are lactose fermenting?
Lactose fermenting species will grow pink colonies. Lactose fermentation will produce acidic byproducts that lower the pH, and this turns the pH indicator to pink. Example of Lac positive species: Escherichia coli, Enterobacteria, Klebsiella.
What is the principle of indole test?
Principle of Indole Test Tryptophanase catalyzes the deamination reaction, during which the amine (-NH2) group of the tryptophan molecule is removed. Final products of the reaction are indole, pyruvic acid, ammonium (NH4+) and energy. Pyridoxal phosphate is required as a coenzyme.
What does a positive Kia test mean?
Positive Test for Butt Reaction – Yellow (acid) Negative Test for Butt Reaction – Red (alkaline) KIA Color Reactions: Red slant/ yellow butt –
What is the principle of MacConkey agar?
Principle of MacConkey Agar Lactose monohydrate is the fermentable source of carbohydrate. The selective action of this medium is attributed to crystal violet and bile salts, which are inhibitory to most species of gram-positive bacteria. Sodium chloride maintains the osmotic balance in the medium.
What kind of biochemical test does Kia do?
It tests for organisms’ abilities to ferment glucose and lactose to acid and acid plus gas end products. It also allows for identification of sulfur reducers.
What kind of reactions do bacteria have on Kia?
Bacteria growing on KIA produces three general types of reactions. No change in the medium i.e. Alkaline Slant/Alkaline Butt (K/K): No carbohydrate fermentation. Presence of non-fermentative gram-negative bacilli (organisms that are unable to produce acids from the fermentation of glucose or lactose) e.g. Pseudomonas aeruginosa.
What do you need to know about Kia?
Kliger’s Iron Agar (KIA) This is a differential medium. It tests for organisms’ abilities to ferment glucose and lactose to acid and acid plus gas end products. It also allows for identification of sulfur reducers.
What is the composition of Kligler’s Iron Agar ( Kia )?
The composition of Kligler’s Iron Agar (KIA) is identical to Triple Sugar Iron Agar (TSI) except that 10 gm sucrose is added in TSI.